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sheep anti gfp eyfp  (Bio-Rad)


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    Bio-Rad sheep anti gfp eyfp
    Sheep Anti Gfp Eyfp, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+gfp+eyfp/Sheep+anti+Green+Fluorescent+Protein/pmc12442953-3-0-4
    Average 95 stars, based on 165 article reviews
    sheep anti gfp eyfp - by Bioz Stars, 2026-10
    95/100 stars

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    Virus:

    Article Title: Intradental mechano-nociceptors serve as sentinels that prevent tooth damage.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-RFP (1:500) Rockland 600-401-379; RRID:AB_2209751 Chicken anti-GFP (1:1000) Life Technologies PA1-9533; RRID:AB_1074893 Sheep anti-GFP/eYFP (1:500) Bio-Rad 4747–1051; RRID:AB_619712 Rat anti-CD45 (1:500) BioLegend 103101; RRID:AB_312966 Goat anti-rabbit (Alexa Fluor 568) Life Technologies A11011; RRID:AB_143157 Goat anti-chicken (Alexa Fluor 647) Life Technologies A21449; RRID:AB_2535866 Donkey anti-sheep (Alexa Fluor 488) Life Technologies A11015; RRID:AB_141362 Donkey anti-rat (Alexa Fluor 647) Invitrogen A48272; RRID:AB_2893138 Bacterial and virus strains ssAAV-9/2-hEF1a-iCre-WPRE-bGHp(A) (Physical titer: 8.1 × 10E12 vg/mL) University of Zurich Viral Vector Facility VVF, Zurich, CH v225-9 ssAAV-9/2-hSyn1-chI-mCherry_2A_iCre-WPRESV40p(A) (Physical titer: 5.6 × 10E12 vg/mL) University of Zurich Viral Vector Facility VVF, Zurich, CH v147-9 AAV9-CAG-FLEX-tdT (Physical titer: 1–9 × 10E13 vg/mL) Vigene Discontinued pAAV-EF1α-Brainbow-invert tagBFP-eYFP-wPRE (Physical titer: 2.2 × 10E13 vg/mL) Addgene #V120025 pAAV-Ef1a-Brainbow/mCherry/mTFPWPRE (Physical titer: 2.2 × 10E13 vg/mL) Addgene #V160749 ssAAV-6(F129L)/2-hEF1a-iCre-WPREbGHp(A) (Physical titer: 7.2 × 10E12 vg/mL) University of Zurich Viral Vector Facility VVF, Zurich, CH #225-6(F129L)/2 Chemicals, peptides, and recombinant proteins Cholera Toxin Subunit B (Recombinant), Alexa Fluor TM 647 Conjugate ThermoFisher C34778 Isofluorane MWI Animal Health 501017 Normal Donkey Serum Millipore Sigma S30-M Normal Goat Serum Millipore Sigma S26-M Paraformaldehyde (PFA) fixative Electron Microscopy Sciences 15714-S 5% silk fibroin Advanced BioMatrix 5154-20ML Flow-It TM ALC TM Pentron Shade B2 Deposited data Custom code for analysis Zenodo https://doi.org/10.5281/zenodo.15643732 Critical commercial assays Hybridization Chain Reaction (HCR) version 3 kit Molecular Instruments N/A RNAscopeTM Multiplex Fluorescent Detection Reagents version 2 Advanced Cell Diagnostics, Inc 323110 Experimental models: Organisms/strains Mouse: Ai95(RCL-GCaMP6f)-D (C57BL/6J) JAX (The Jackson Laboratory) Stock no. 028865; RRID:IMSR_JAX:028865 Mouse: Piezo2 lox/lox ; Tac1-tagRFP-2a-TVA line Szczot et al. 21 N/A Mouse: Snap25-LSL-2A-EGFP-D JAX Stock no. 021879; RRID:IMSR_JAX:021879 Mouse: Na v 1.8-Cre JAX Stock no. 036564; RRID:IMSR_JAX:036564 Mouse: CAG-LSL-Gq-DREADD JAX Stock no. 026220; RRID:IMSR_JAX:026220 Mouse: C57BL/6J JAX Stock no. 000664; RRID:IMSR_JAX:000664 (Continued on next page) 14 Cell Reports 44, 116017, August 26, 2025 ..

    Recombinant:

    Article Title: Intradental mechano-nociceptors serve as sentinels that prevent tooth damage.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-RFP (1:500) Rockland 600-401-379; RRID:AB_2209751 Chicken anti-GFP (1:1000) Life Technologies PA1-9533; RRID:AB_1074893 Sheep anti-GFP/eYFP (1:500) Bio-Rad 4747–1051; RRID:AB_619712 Rat anti-CD45 (1:500) BioLegend 103101; RRID:AB_312966 Goat anti-rabbit (Alexa Fluor 568) Life Technologies A11011; RRID:AB_143157 Goat anti-chicken (Alexa Fluor 647) Life Technologies A21449; RRID:AB_2535866 Donkey anti-sheep (Alexa Fluor 488) Life Technologies A11015; RRID:AB_141362 Donkey anti-rat (Alexa Fluor 647) Invitrogen A48272; RRID:AB_2893138 Bacterial and virus strains ssAAV-9/2-hEF1a-iCre-WPRE-bGHp(A) (Physical titer: 8.1 × 10E12 vg/mL) University of Zurich Viral Vector Facility VVF, Zurich, CH v225-9 ssAAV-9/2-hSyn1-chI-mCherry_2A_iCre-WPRESV40p(A) (Physical titer: 5.6 × 10E12 vg/mL) University of Zurich Viral Vector Facility VVF, Zurich, CH v147-9 AAV9-CAG-FLEX-tdT (Physical titer: 1–9 × 10E13 vg/mL) Vigene Discontinued pAAV-EF1α-Brainbow-invert tagBFP-eYFP-wPRE (Physical titer: 2.2 × 10E13 vg/mL) Addgene #V120025 pAAV-Ef1a-Brainbow/mCherry/mTFPWPRE (Physical titer: 2.2 × 10E13 vg/mL) Addgene #V160749 ssAAV-6(F129L)/2-hEF1a-iCre-WPREbGHp(A) (Physical titer: 7.2 × 10E12 vg/mL) University of Zurich Viral Vector Facility VVF, Zurich, CH #225-6(F129L)/2 Chemicals, peptides, and recombinant proteins Cholera Toxin Subunit B (Recombinant), Alexa Fluor TM 647 Conjugate ThermoFisher C34778 Isofluorane MWI Animal Health 501017 Normal Donkey Serum Millipore Sigma S30-M Normal Goat Serum Millipore Sigma S26-M Paraformaldehyde (PFA) fixative Electron Microscopy Sciences 15714-S 5% silk fibroin Advanced BioMatrix 5154-20ML Flow-It TM ALC TM Pentron Shade B2 Deposited data Custom code for analysis Zenodo https://doi.org/10.5281/zenodo.15643732 Critical commercial assays Hybridization Chain Reaction (HCR) version 3 kit Molecular Instruments N/A RNAscopeTM Multiplex Fluorescent Detection Reagents version 2 Advanced Cell Diagnostics, Inc 323110 Experimental models: Organisms/strains Mouse: Ai95(RCL-GCaMP6f)-D (C57BL/6J) JAX (The Jackson Laboratory) Stock no. 028865; RRID:IMSR_JAX:028865 Mouse: Piezo2 lox/lox ; Tac1-tagRFP-2a-TVA line Szczot et al. 21 N/A Mouse: Snap25-LSL-2A-EGFP-D JAX Stock no. 021879; RRID:IMSR_JAX:021879 Mouse: Na v 1.8-Cre JAX Stock no. 036564; RRID:IMSR_JAX:036564 Mouse: CAG-LSL-Gq-DREADD JAX Stock no. 026220; RRID:IMSR_JAX:026220 Mouse: C57BL/6J JAX Stock no. 000664; RRID:IMSR_JAX:000664 (Continued on next page) 14 Cell Reports 44, 116017, August 26, 2025 ..

    Electron Microscopy:

    Article Title: Intradental mechano-nociceptors serve as sentinels that prevent tooth damage.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-RFP (1:500) Rockland 600-401-379; RRID:AB_2209751 Chicken anti-GFP (1:1000) Life Technologies PA1-9533; RRID:AB_1074893 Sheep anti-GFP/eYFP (1:500) Bio-Rad 4747–1051; RRID:AB_619712 Rat anti-CD45 (1:500) BioLegend 103101; RRID:AB_312966 Goat anti-rabbit (Alexa Fluor 568) Life Technologies A11011; RRID:AB_143157 Goat anti-chicken (Alexa Fluor 647) Life Technologies A21449; RRID:AB_2535866 Donkey anti-sheep (Alexa Fluor 488) Life Technologies A11015; RRID:AB_141362 Donkey anti-rat (Alexa Fluor 647) Invitrogen A48272; RRID:AB_2893138 Bacterial and virus strains ssAAV-9/2-hEF1a-iCre-WPRE-bGHp(A) (Physical titer: 8.1 × 10E12 vg/mL) University of Zurich Viral Vector Facility VVF, Zurich, CH v225-9 ssAAV-9/2-hSyn1-chI-mCherry_2A_iCre-WPRESV40p(A) (Physical titer: 5.6 × 10E12 vg/mL) University of Zurich Viral Vector Facility VVF, Zurich, CH v147-9 AAV9-CAG-FLEX-tdT (Physical titer: 1–9 × 10E13 vg/mL) Vigene Discontinued pAAV-EF1α-Brainbow-invert tagBFP-eYFP-wPRE (Physical titer: 2.2 × 10E13 vg/mL) Addgene #V120025 pAAV-Ef1a-Brainbow/mCherry/mTFPWPRE (Physical titer: 2.2 × 10E13 vg/mL) Addgene #V160749 ssAAV-6(F129L)/2-hEF1a-iCre-WPREbGHp(A) (Physical titer: 7.2 × 10E12 vg/mL) University of Zurich Viral Vector Facility VVF, Zurich, CH #225-6(F129L)/2 Chemicals, peptides, and recombinant proteins Cholera Toxin Subunit B (Recombinant), Alexa Fluor TM 647 Conjugate ThermoFisher C34778 Isofluorane MWI Animal Health 501017 Normal Donkey Serum Millipore Sigma S30-M Normal Goat Serum Millipore Sigma S26-M Paraformaldehyde (PFA) fixative Electron Microscopy Sciences 15714-S 5% silk fibroin Advanced BioMatrix 5154-20ML Flow-It TM ALC TM Pentron Shade B2 Deposited data Custom code for analysis Zenodo https://doi.org/10.5281/zenodo.15643732 Critical commercial assays Hybridization Chain Reaction (HCR) version 3 kit Molecular Instruments N/A RNAscopeTM Multiplex Fluorescent Detection Reagents version 2 Advanced Cell Diagnostics, Inc 323110 Experimental models: Organisms/strains Mouse: Ai95(RCL-GCaMP6f)-D (C57BL/6J) JAX (The Jackson Laboratory) Stock no. 028865; RRID:IMSR_JAX:028865 Mouse: Piezo2 lox/lox ; Tac1-tagRFP-2a-TVA line Szczot et al. 21 N/A Mouse: Snap25-LSL-2A-EGFP-D JAX Stock no. 021879; RRID:IMSR_JAX:021879 Mouse: Na v 1.8-Cre JAX Stock no. 036564; RRID:IMSR_JAX:036564 Mouse: CAG-LSL-Gq-DREADD JAX Stock no. 026220; RRID:IMSR_JAX:026220 Mouse: C57BL/6J JAX Stock no. 000664; RRID:IMSR_JAX:000664 (Continued on next page) 14 Cell Reports 44, 116017, August 26, 2025 ..

    Hybridization:

    Article Title: Intradental mechano-nociceptors serve as sentinels that prevent tooth damage.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-RFP (1:500) Rockland 600-401-379; RRID:AB_2209751 Chicken anti-GFP (1:1000) Life Technologies PA1-9533; RRID:AB_1074893 Sheep anti-GFP/eYFP (1:500) Bio-Rad 4747–1051; RRID:AB_619712 Rat anti-CD45 (1:500) BioLegend 103101; RRID:AB_312966 Goat anti-rabbit (Alexa Fluor 568) Life Technologies A11011; RRID:AB_143157 Goat anti-chicken (Alexa Fluor 647) Life Technologies A21449; RRID:AB_2535866 Donkey anti-sheep (Alexa Fluor 488) Life Technologies A11015; RRID:AB_141362 Donkey anti-rat (Alexa Fluor 647) Invitrogen A48272; RRID:AB_2893138 Bacterial and virus strains ssAAV-9/2-hEF1a-iCre-WPRE-bGHp(A) (Physical titer: 8.1 × 10E12 vg/mL) University of Zurich Viral Vector Facility VVF, Zurich, CH v225-9 ssAAV-9/2-hSyn1-chI-mCherry_2A_iCre-WPRESV40p(A) (Physical titer: 5.6 × 10E12 vg/mL) University of Zurich Viral Vector Facility VVF, Zurich, CH v147-9 AAV9-CAG-FLEX-tdT (Physical titer: 1–9 × 10E13 vg/mL) Vigene Discontinued pAAV-EF1α-Brainbow-invert tagBFP-eYFP-wPRE (Physical titer: 2.2 × 10E13 vg/mL) Addgene #V120025 pAAV-Ef1a-Brainbow/mCherry/mTFPWPRE (Physical titer: 2.2 × 10E13 vg/mL) Addgene #V160749 ssAAV-6(F129L)/2-hEF1a-iCre-WPREbGHp(A) (Physical titer: 7.2 × 10E12 vg/mL) University of Zurich Viral Vector Facility VVF, Zurich, CH #225-6(F129L)/2 Chemicals, peptides, and recombinant proteins Cholera Toxin Subunit B (Recombinant), Alexa Fluor TM 647 Conjugate ThermoFisher C34778 Isofluorane MWI Animal Health 501017 Normal Donkey Serum Millipore Sigma S30-M Normal Goat Serum Millipore Sigma S26-M Paraformaldehyde (PFA) fixative Electron Microscopy Sciences 15714-S 5% silk fibroin Advanced BioMatrix 5154-20ML Flow-It TM ALC TM Pentron Shade B2 Deposited data Custom code for analysis Zenodo https://doi.org/10.5281/zenodo.15643732 Critical commercial assays Hybridization Chain Reaction (HCR) version 3 kit Molecular Instruments N/A RNAscopeTM Multiplex Fluorescent Detection Reagents version 2 Advanced Cell Diagnostics, Inc 323110 Experimental models: Organisms/strains Mouse: Ai95(RCL-GCaMP6f)-D (C57BL/6J) JAX (The Jackson Laboratory) Stock no. 028865; RRID:IMSR_JAX:028865 Mouse: Piezo2 lox/lox ; Tac1-tagRFP-2a-TVA line Szczot et al. 21 N/A Mouse: Snap25-LSL-2A-EGFP-D JAX Stock no. 021879; RRID:IMSR_JAX:021879 Mouse: Na v 1.8-Cre JAX Stock no. 036564; RRID:IMSR_JAX:036564 Mouse: CAG-LSL-Gq-DREADD JAX Stock no. 026220; RRID:IMSR_JAX:026220 Mouse: C57BL/6J JAX Stock no. 000664; RRID:IMSR_JAX:000664 (Continued on next page) 14 Cell Reports 44, 116017, August 26, 2025 ..

    Multiplex Assay:

    Article Title: Intradental mechano-nociceptors serve as sentinels that prevent tooth damage.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-RFP (1:500) Rockland 600-401-379; RRID:AB_2209751 Chicken anti-GFP (1:1000) Life Technologies PA1-9533; RRID:AB_1074893 Sheep anti-GFP/eYFP (1:500) Bio-Rad 4747–1051; RRID:AB_619712 Rat anti-CD45 (1:500) BioLegend 103101; RRID:AB_312966 Goat anti-rabbit (Alexa Fluor 568) Life Technologies A11011; RRID:AB_143157 Goat anti-chicken (Alexa Fluor 647) Life Technologies A21449; RRID:AB_2535866 Donkey anti-sheep (Alexa Fluor 488) Life Technologies A11015; RRID:AB_141362 Donkey anti-rat (Alexa Fluor 647) Invitrogen A48272; RRID:AB_2893138 Bacterial and virus strains ssAAV-9/2-hEF1a-iCre-WPRE-bGHp(A) (Physical titer: 8.1 × 10E12 vg/mL) University of Zurich Viral Vector Facility VVF, Zurich, CH v225-9 ssAAV-9/2-hSyn1-chI-mCherry_2A_iCre-WPRESV40p(A) (Physical titer: 5.6 × 10E12 vg/mL) University of Zurich Viral Vector Facility VVF, Zurich, CH v147-9 AAV9-CAG-FLEX-tdT (Physical titer: 1–9 × 10E13 vg/mL) Vigene Discontinued pAAV-EF1α-Brainbow-invert tagBFP-eYFP-wPRE (Physical titer: 2.2 × 10E13 vg/mL) Addgene #V120025 pAAV-Ef1a-Brainbow/mCherry/mTFPWPRE (Physical titer: 2.2 × 10E13 vg/mL) Addgene #V160749 ssAAV-6(F129L)/2-hEF1a-iCre-WPREbGHp(A) (Physical titer: 7.2 × 10E12 vg/mL) University of Zurich Viral Vector Facility VVF, Zurich, CH #225-6(F129L)/2 Chemicals, peptides, and recombinant proteins Cholera Toxin Subunit B (Recombinant), Alexa Fluor TM 647 Conjugate ThermoFisher C34778 Isofluorane MWI Animal Health 501017 Normal Donkey Serum Millipore Sigma S30-M Normal Goat Serum Millipore Sigma S26-M Paraformaldehyde (PFA) fixative Electron Microscopy Sciences 15714-S 5% silk fibroin Advanced BioMatrix 5154-20ML Flow-It TM ALC TM Pentron Shade B2 Deposited data Custom code for analysis Zenodo https://doi.org/10.5281/zenodo.15643732 Critical commercial assays Hybridization Chain Reaction (HCR) version 3 kit Molecular Instruments N/A RNAscopeTM Multiplex Fluorescent Detection Reagents version 2 Advanced Cell Diagnostics, Inc 323110 Experimental models: Organisms/strains Mouse: Ai95(RCL-GCaMP6f)-D (C57BL/6J) JAX (The Jackson Laboratory) Stock no. 028865; RRID:IMSR_JAX:028865 Mouse: Piezo2 lox/lox ; Tac1-tagRFP-2a-TVA line Szczot et al. 21 N/A Mouse: Snap25-LSL-2A-EGFP-D JAX Stock no. 021879; RRID:IMSR_JAX:021879 Mouse: Na v 1.8-Cre JAX Stock no. 036564; RRID:IMSR_JAX:036564 Mouse: CAG-LSL-Gq-DREADD JAX Stock no. 026220; RRID:IMSR_JAX:026220 Mouse: C57BL/6J JAX Stock no. 000664; RRID:IMSR_JAX:000664 (Continued on next page) 14 Cell Reports 44, 116017, August 26, 2025 ..



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    97
    Cell Signaling Technology Inc chr2 eyfp
    A, Schematic of mouse models: two doses of tamoxifen (3 mg × 2) were injected intraperitoneally with a 48-hour interval. B, Representative immunofluorescence <t>(IF)</t> <t>staining</t> of <t>EYFP</t> in stomach tissues from Tmprss2-CreER T2 ;Rosa26-LSL-EYFP mice . Nuclei were counterstained with DAPI. Scale bar, 200 µm. C, Kaplan-Meier plots showing the survival of mice after gene knockout. D, Stomach weight of the indicated genetically engineered mouse model (GEMM) six weeks after tamoxifen injection. Data are presented as mean ± SD and analyzed with two-tailed t-test. E, Thickness in corpus mucosa measured by microscopy of hematoxylin and eosin (H&E) staining section. Each dot represents the averaged thickness of 5 random fields from one mouse. Data are presented as mean ± SD and analyzed with two-tailed t-test. F, Representative H&E staining of stomach tissues after tamoxifen administration. Scale bar, 200 µm. G, Pathological staging of stomach cancer progression based on infiltration of tumor cells in TPCD mice. H, Representative H&E staining of small and large intestines in TPCD mice. Scale bar, 1 mm.
    Chr2 Eyfp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A, Schematic of mouse models: two doses of tamoxifen (3 mg × 2) were injected intraperitoneally with a 48-hour interval. B, Representative immunofluorescence (IF) staining of EYFP in stomach tissues from Tmprss2-CreER T2 ;Rosa26-LSL-EYFP mice . Nuclei were counterstained with DAPI. Scale bar, 200 µm. C, Kaplan-Meier plots showing the survival of mice after gene knockout. D, Stomach weight of the indicated genetically engineered mouse model (GEMM) six weeks after tamoxifen injection. Data are presented as mean ± SD and analyzed with two-tailed t-test. E, Thickness in corpus mucosa measured by microscopy of hematoxylin and eosin (H&E) staining section. Each dot represents the averaged thickness of 5 random fields from one mouse. Data are presented as mean ± SD and analyzed with two-tailed t-test. F, Representative H&E staining of stomach tissues after tamoxifen administration. Scale bar, 200 µm. G, Pathological staging of stomach cancer progression based on infiltration of tumor cells in TPCD mice. H, Representative H&E staining of small and large intestines in TPCD mice. Scale bar, 1 mm.

    Journal: bioRxiv

    Article Title: Loss of Kmt2c / d promotes gastric cancer initiation and confers vulnerability to mTORC1 inhibition and anti-PD1 immunotherapy

    doi: 10.1101/2025.03.27.645747

    Figure Lengend Snippet: A, Schematic of mouse models: two doses of tamoxifen (3 mg × 2) were injected intraperitoneally with a 48-hour interval. B, Representative immunofluorescence (IF) staining of EYFP in stomach tissues from Tmprss2-CreER T2 ;Rosa26-LSL-EYFP mice . Nuclei were counterstained with DAPI. Scale bar, 200 µm. C, Kaplan-Meier plots showing the survival of mice after gene knockout. D, Stomach weight of the indicated genetically engineered mouse model (GEMM) six weeks after tamoxifen injection. Data are presented as mean ± SD and analyzed with two-tailed t-test. E, Thickness in corpus mucosa measured by microscopy of hematoxylin and eosin (H&E) staining section. Each dot represents the averaged thickness of 5 random fields from one mouse. Data are presented as mean ± SD and analyzed with two-tailed t-test. F, Representative H&E staining of stomach tissues after tamoxifen administration. Scale bar, 200 µm. G, Pathological staging of stomach cancer progression based on infiltration of tumor cells in TPCD mice. H, Representative H&E staining of small and large intestines in TPCD mice. Scale bar, 1 mm.

    Article Snippet: Immunofluorescent staining was performed using primary antibodies against EYFP (2956, Cell Signaling Technology, 1:100), H3K4me1 (5326, Cell Signaling Technology, 1:100), ATP4A (D031-3, MBL Life Science, 1:500), and Puromycin (MABE343, Millipore Sigma, 1:100).

    Techniques: Injection, Immunofluorescence, Staining, Gene Knockout, Two Tailed Test, Microscopy